Difference between revisions of "Part:BBa I725099"

Latest revision as of 21:59, 2 May 2008

Partial operon for butanoate pathway

The butanoate pathway in Clostridium acetobutylicum from acetyl-CoA to butanol requires five (or six) enzymes. In order, these are acetyl-CoA acetyltransferase (thiolase in E. coli), B-hydroxy butyryl coA dehydrogenase, Enoyl-coA hydratase (from E. coli), Butyryl coA Dehydrogenase, Butyraldehyde dehydrogenase, and Butanol dehydrogenase. It is unclear whether the E. coli thiolase will be sufficient for butanol production in E. coli or whether overexpression will be required. Subsequently, it is not included in this version of the operon.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 308
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 763
Illegal AgeI site found at 1077
Illegal AgeI site found at 1208
Illegal AgeI site found at 1988
Illegal AgeI site found at 3091
Illegal AgeI site found at 3493
Illegal AgeI site found at 4717
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_I725099 short</partinfo>
-The butanoate pathway in Clostridium acetobutylicum from acetyl-CoA to butanol requires five (or six) enzymes. In order, these are acetyl-CoA acetyltransferase (thiolase in E. coli), B-hydroxy butyryl coA dehydrogenase, Enoyl-coA hydratase (from E. coli), Butyryl coA Dehydrogenase, Butyraldehyde dehydrogenase, and Butanol dehydrogenase. It is unclear whether the E. coli thiolase will be sufficient for butanol production in E. coli or whether overexpression will be required. Subsequently, it is not included in this version of the operon.
+The butanoate pathway in ''Clostridium acetobutylicum'' from acetyl-CoA to butanol requires five (or six) enzymes. In order, these are acetyl-CoA acetyltransferase (thiolase in ''E. coli''), B-hydroxy butyryl coA dehydrogenase, Enoyl-coA hydratase (from ''E. coli''), Butyryl coA Dehydrogenase, Butyraldehyde dehydrogenase, and Butanol dehydrogenase. It is unclear whether the ''E. coli'' thiolase will be sufficient for butanol production in ''E. coli'' or whether overexpression will be required. Subsequently, it is not included in this version of the operon.