Difference between revisions of "Part:BBa K2610032"
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We, iGEM Leiden 2018, have used confocal microscopy in order to visualize the E.coli stress response to nalidixic acid. We incubated our transformed DH5a cells with 100 ug/mL nalidixic acid for 3 hours, after which they were analysed with the confocal (Figure 1). As can be observed, treating the cells with nalidixic acid resulted in increased fluorescence. | We, iGEM Leiden 2018, have used confocal microscopy in order to visualize the E.coli stress response to nalidixic acid. We incubated our transformed DH5a cells with 100 ug/mL nalidixic acid for 3 hours, after which they were analysed with the confocal (Figure 1). As can be observed, treating the cells with nalidixic acid resulted in increased fluorescence. | ||
| + | [[File:T--Leiden--SoxS-mcherry.png|750px]] <br> | ||
| + | <span style="font-size:1em"><b>Figure 1.</b> pSoxS-mCherry transformed cells untreated (A and B) and treated with 100 ug/mL nalidixic acid. </span> | ||
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Revision as of 08:02, 10 October 2018
pSoxS-mCherry
This composite part features the regulatory part promoter SoxS (BBa_K2610030) and the fluorescent protein mCherry (BBa_K180008). It can be used to visualize upregulation of SoxS as a result of superoxide stress.
Regulatory protein SoxS is involved in the superoxide pathway in Escherichia coli. It acts a superactivator of downstream stress genes. We have coupled this promoter to mCherry, a second generation monomeric fluorescent protein.
Usage and Biology
We, iGEM Leiden 2018, have used confocal microscopy in order to visualize the E.coli stress response to nalidixic acid. We incubated our transformed DH5a cells with 100 ug/mL nalidixic acid for 3 hours, after which they were analysed with the confocal (Figure 1). As can be observed, treating the cells with nalidixic acid resulted in increased fluorescence.
Figure 1. pSoxS-mCherry transformed cells untreated (A and B) and treated with 100 ug/mL nalidixic acid.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]