Difference between revisions of "Part:BBa K2610031"
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===Usage and Biology===
 
===Usage and Biology===
  
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We, iGEM Leiden 2018, have designed this composite part as part of our project Fifty Shades of Stress. This reporter part allowed us to detect stress-induced changes in SoxS transcription.  
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We, iGEM Leiden 2018, have designed this composite part as part of our project Fifty Shades of Stress. This reporter part allowed us to detect stress-induced changes in SoxS transcription using flow cytometry.  
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As can be seen in figure 1, treatment with increasing concentrations of nalidixic acid leads to an increasing median fluorescence. 
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As can be seen in figure 1, treatment with increasing concentrations of nalidixic acid leads to an increasing median fluorescence. Moreover, we have integrated this promoter into a heatmap with many stress-related promoters (Figure 2). SoxS is activated specifically by nalidixic acid and hydrogen peroxide.
  
 
[[File:T--Leiden--SoxS-GFP.png|750px]] <br>
 
[[File:T--Leiden--SoxS-GFP.png|750px]] <br>
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<span style="font-size:1em"><b>Figure 1.</b> Median Fluorescence Intensity (MFI) in AU after 4 hour incubation with nalidixic acid in various concentrations. </span>  
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<span style="font-size:1em"><b>Figure 2.</b> Median Fluorescence Intensity (MFI) in AU after 4 hour incubation with nalidixic acid in various concentrations. </span>  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 07:57, 10 October 2018


pSoxS-GFP

This composite part features the regulatory part promoter SoxS (BBa_K2610030) and the fluorescent protein GFP (BBa_E0040). It can be used to visualize upregulation of SoxS as a result of superoxide stress.

Regulatory protein SoxS is involved in the superoxide pathway in Escherichia coli. It acts a superactivator of downstream stress genes.

Usage and Biology

We, iGEM Leiden 2018, have designed this composite part as part of our project Fifty Shades of Stress. This reporter part allowed us to detect stress-induced changes in SoxS transcription using flow cytometry.

As can be seen in figure 1, treatment with increasing concentrations of nalidixic acid leads to an increasing median fluorescence. Moreover, we have integrated this promoter into a heatmap with many stress-related promoters (Figure 2). SoxS is activated specifically by nalidixic acid and hydrogen peroxide.

T--Leiden--SoxS-GFP.png
Figure 2. Median Fluorescence Intensity (MFI) in AU after 4 hour incubation with nalidixic acid in various concentrations.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 947