Difference between revisions of "Part:BBa K2666001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | We used a strong promoter to produce a lot of LL-37. Lactobacillus is not supposed to be sensitive to LL-37 (reference). | |
| − | + | ||
| + | We added spacers to all of our constructions to unable easier use of the sequence and separation of the different genes of the sequences. We used two Terminators to our sequences :BBa_B0014 & BBa_B0015 to ensure the stopping of the transcription. | ||
===Source=== | ===Source=== | ||
Latest revision as of 07:44, 10 October 2018
LL-37 with L.jensenii specific strong promoter RpsU
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 62
Illegal BamHI site found at 194 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We used a strong promoter to produce a lot of LL-37. Lactobacillus is not supposed to be sensitive to LL-37 (reference).
We added spacers to all of our constructions to unable easier use of the sequence and separation of the different genes of the sequences. We used two Terminators to our sequences :BBa_B0014 & BBa_B0015 to ensure the stopping of the transcription.
Source
References
[1] Bao, Sujin, et al. "Distribution dynamics of recombinant Lactobacillus in the gastrointestinal tract of neonatal rats." PloS one 8.3 (2013): e60007.
[2] Tanphaichitr, Nongnuj et al. 2018. Potential Use of Antimicrobial Peptides as Vaginal Spermicides/Microbicides. Pharmaceuticals 9.1 (2016): 13.