Difference between revisions of "Part:BBa K2570002"

Revision as of 07:41, 10 October 2018

TyrB

phenyllactic acid（PLA）is widely found in kimchi, honey and other foods. It is a new type of natural antibacterial substance and preservative, which can inhibit a series of gram-negative, gram-positive bacteria and fungi. In addition, PLA has obvious improvement in protection of the cardiovascular system and has been widely used in the food and pharmaceutical industries. In order to enhance the production of PLA, we expressed D-lactate dehydrogenase BBa_K257000 and phenylalanine aminotransferase BBa_K2570002 and expressed rocG BBa_K2570004 to introduce the cofactor circulatory system to optimize the transformation system conditions and use phenylalanine as a substrate.

Production of PLA.

This sequence encodes for Phenylalanine aminotransferase Tyrb that is one of the two key enzymes in the anabolic pathway of phenyllactic acid biosynthesis. Phenylalanine aminotransferase (PheATs), also known as aromatic transaminase (AroATs) or tyrosine aminotransferase (TyrATs), is the key enzyme responsible for formation of the compound pyridoxa-l-5'- (PLP), and is also essential for the biosynthesis of phenylalanine and tyrosinase. We used phenylalanine transaminase from Escherichia coli 21B to deaminate the substrate phenylalanine combined with aminotransferase to produce phenylpyruvic acid that is the raw material of the target product PLA.

Our project uses phenylalanine aminotransferase (PAT) to catalyze the substrate phenylalanine aminotransferase to produce more intermediate metabolite phenylpyruvic acid, which can be transformed to D-PLA by D-lactate dehydrogenase BBa_K2570018.

Sequence and Features