Difference between revisions of "Part:BBa K2872881"
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<partinfo>BBa_K2872881 short</partinfo> | <partinfo>BBa_K2872881 short</partinfo> | ||
| − | This is the activating sequence of Cas12a. Upon gRNA-directed binding of Cas12a to this sequence, there is an indiscriminate degradation of DNA by Cas12a | + | This is the activating sequence of Cas12a. Upon gRNA-directed binding of Cas12a to this sequence, there is an indiscriminate degradation of DNA by Cas12a. |
| + | Sequence information was first obtained using OMIM: https://www.omim.org/entry/141900?search=rs334&highlight=rs334 | ||
| + | |||
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Latest revision as of 07:41, 10 October 2018
Template DNA sequence coding for HBB rs334 mutation (A to T) in humans, PAM site added
This is the activating sequence of Cas12a. Upon gRNA-directed binding of Cas12a to this sequence, there is an indiscriminate degradation of DNA by Cas12a. Sequence information was first obtained using OMIM: https://www.omim.org/entry/141900?search=rs334&highlight=rs334
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 178