Difference between revisions of "Part:BBa K2610030"
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| − | Regulatory protein SoxS is involved in the superoxide pathway in Escherichia coli. It acts a superactivator of downstream stress genes | + | Regulatory protein SoxS is involved in the superoxide pathway in Escherichia coli. It acts a superactivator of downstream stress genes. |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 07:38, 10 October 2018
pSoxS
Regulatory protein SoxS is involved in the superoxide pathway in Escherichia coli. It acts a superactivator of downstream stress genes.
Usage and Biology
iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) using flow cytometry. We fused promoters to green fluorescent protein and stressed transformed DH5a cells for several hours.
Figure 1. Heatmap with stress-activated promoters
As can be observed in Figure 1 the SoxS promoter is specifically activated by nalidixic acid and hydrogen peroxide.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]