Difference between revisions of "Part:BBa K2668070:Design"

 
 
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===Design Notes===
 
===Design Notes===
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in fusion cloning in pGAP and pETDuet1
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The part BBa_K2668070 comes from the <a href="http://2018.igem.org/Team:Toulouse-INSA-UPS/Design">Cerberus Project</a>. It was design to validate the Orthos part (i.e. fusion protein between CBM3a and a monomeric streptavidin head).
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===Source===
 
===Source===
  
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IDT gBlock
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The part was obtained by synthesis by IDT as gBlock, The mTag Blue Fluorescent Protein is Part:BBa_K592100 and was fused with an Avi Tag (Fairhead et Howarth 2015).
  
 
===References===
 
===References===
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Fairhead M, Howarth M. Site-specific biotinylation of purified proteins using BirA. Site-Specific Protein Labeling: Methods and Protocols. 2015. pp. 171–184

Latest revision as of 21:49, 9 October 2018


mTagBFP AviTag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part BBa_K2668070 comes from the Cerberus Project. It was design to validate the Orthos part (i.e. fusion protein between CBM3a and a monomeric streptavidin head).

Source

The part was obtained by synthesis by IDT as gBlock, The mTag Blue Fluorescent Protein is Part:BBa_K592100 and was fused with an Avi Tag (Fairhead et Howarth 2015).

References

Fairhead M, Howarth M. Site-specific biotinylation of purified proteins using BirA. Site-Specific Protein Labeling: Methods and Protocols. 2015. pp. 171–184