Difference between revisions of "Part:BBa K2614000:Design"

 
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===Design Notes===
 
===Design Notes===
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We amplified the template by a PCR and digested with EcoRI an PstI. Subsequently we digested the pSB1C3 plasmid with EcoRI and PstI and ligated the digested Plasmid with our digested template.
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We amplifiy the template by a PCR and digest it with EcoRI-HF an PstI-HF. Subsequently we digest the pSB1C3 plasmid with EcoRI-HF and PstI-HF and ligate the digested Plasmid with our digested template.
  
  
  
 
===Source===
 
===Source===
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As template we used a synthetic oligonucleotide.  
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The template was syntesized by a company.  
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===References===
 
===References===

Revision as of 19:31, 9 October 2018


template as positive control for a qPCR that detects Plasmodium


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We amplifiy the template by a PCR and digest it with EcoRI-HF an PstI-HF. Subsequently we digest the pSB1C3 plasmid with EcoRI-HF and PstI-HF and ligate the digested Plasmid with our digested template.


Source

As template we used a synthetic oligonucleotide.

References