Difference between revisions of "Part:BBa K2842710"

 
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E. coli nickel binding protein, NikR, assembled into BBa_K2842669 (Intein Passenger).
 
E. coli nickel binding protein, NikR, assembled into BBa_K2842669 (Intein Passenger).
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[[File:T--UCL--IP-NikR and IP-ER gel.png|400px|thumb|left|
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<center>'''Figure 1: colony PCR of functionalised Intein Passenger [https://parts.igem.org/Part:BBa_K2842669 BBa_K2842669]'''</center>
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<p>
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(1)  ER protein with TerL-C intein [https://parts.igem.org/BBa_K2842720 BBa_K2842720]<br />
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(2) NikR with TerL-C intein [https://parts.igem.org/BBa_K2842710 BBa_K2842710] <br />
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*edited to show relevant bands
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</p>''' ]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 16:41, 9 October 2018


NikR with TerL-C intein

E. coli nickel binding protein, NikR, assembled into BBa_K2842669 (Intein Passenger).

Figure 1: colony PCR of functionalised Intein Passenger BBa_K2842669

(1) ER protein with TerL-C intein BBa_K2842720
(2) NikR with TerL-C intein BBa_K2842710
*edited to show relevant bands

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 771
    Illegal BsaI.rc site found at 28