Difference between revisions of "Part:BBa I715054"

Latest revision as of 17:41, 23 March 2008

Test Trp anti-terminator/terminator

This part is designed to test the functionality of the trp operon regulatory sequence which uses transcriptional attenuation (via the "anti-termination" mechanism) to regulate gene expression.

Usage and Biology

In the presence of tryptophan, the trp regulatory sequence will attenuate transcription, and GFP will not be transcribed. This construct serves as a means of testing the functionality of the trp regulatory sequence by comparing GFP expression with different environmental concentrations of tryptophan amino acid.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1012

@@ Line 6: / Line 6: @@
 <!-- Add more about the biology of this part here -->
 ===Usage and Biology===
-In the presence of tryptophan, the <i>trp</i> regulatory sequence will attenuate transcription, and GFP will not be transcribed.  This construct serves as a means of testing the functionality of the 'trp' regulatory sequence by comparing GFP expression with different environmental concentrations of tryptophan amino acid.
+In the presence of tryptophan, the <i>trp</i> regulatory sequence will attenuate transcription, and GFP will not be transcribed.  This construct serves as a means of testing the functionality of the <i>trp</i> regulatory sequence by comparing GFP expression with different environmental concentrations of tryptophan amino acid.
 <!-- -->
@@ Line 13: / Line 13: @@
 <!-- Uncomment this to enable Functional Parameter display -->
 ===Functional Parameters===
 <partinfo>BBa_I715054 parameters</partinfo>
 <!-- -->