Difference between revisions of "Part:BBa K2587009"
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<partinfo>BBa_K2587009 short</partinfo> | <partinfo>BBa_K2587009 short</partinfo> | ||
| − | <p>Most of the microorganisms, especially common used model organsims like <i>Escherichia coli</i> or <i>Saccharomyces cerevisiae</i> grow only very slow on phosphite as phosphorus source. Moreover in industry is not widely accepted to use antibiotic resistances as markers. Therefore a system is required, which allows avoidance of contamination by other microorganisms and at the same time represents a reliable selection marker. We present the use of the ptxD gene from <i>Pseudomonas stutzeri</i> together with a phosphite media, which could abolish the use of antibiotics in the future. </p> | + | <p>Most of the microorganisms, especially common used model organsims like <i>Escherichia coli</i> or <i>Saccharomyces cerevisiae</i> grow only very slow on phosphite as phosphorus source. Moreover in industry is not widely accepted to use antibiotic resistances as markers. Therefore a system is required, which allows avoidance of contamination by other microorganisms and at the same time represents a reliable selection marker. We present the use of the ptxD gene from <i>Pseudomonas stutzeri</i> together with a <strong>phosphite media</strong> (reduces growth of contaminants), which could abolish the use of antibiotics in the future and the more efficient use of a phosphorus source in the medium. In this case ptxD is codon optimised for <i>S.cerevisiae</i>.</p> |
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<p><ul> | <p><ul> | ||
| − | <li | + | <li> ptxD codes for phosphonate dehydrogenase</li> |
| − | <li | + | <li> oxidation of phosphite (phosphonate) using NAD+ and H20 to phosphate and NADH </li> |
| − | <li | + | <li> Selection marker for budding and fission yeast</li> |
| − | <li | + | <li> phosphite-oxidizing ability </li> |
| − | <li | + | <li> environmentally safe culture</li> |
<li> <strong>antibiotic free system</strong></li> | <li> <strong>antibiotic free system</strong></li> | ||
<li> <strong> pH optimum: 7.25- 7.75</strong></li> | <li> <strong> pH optimum: 7.25- 7.75</strong></li> | ||
Revision as of 15:50, 9 October 2018
ptxD_opt
Most of the microorganisms, especially common used model organsims like Escherichia coli or Saccharomyces cerevisiae grow only very slow on phosphite as phosphorus source. Moreover in industry is not widely accepted to use antibiotic resistances as markers. Therefore a system is required, which allows avoidance of contamination by other microorganisms and at the same time represents a reliable selection marker. We present the use of the ptxD gene from Pseudomonas stutzeri together with a phosphite media (reduces growth of contaminants), which could abolish the use of antibiotics in the future and the more efficient use of a phosphorus source in the medium. In this case ptxD is codon optimised for S.cerevisiae.
Usage and Biology
- ptxD codes for phosphonate dehydrogenase
- oxidation of phosphite (phosphonate) using NAD+ and H20 to phosphate and NADH
- Selection marker for budding and fission yeast
- phosphite-oxidizing ability
- environmentally safe culture
- antibiotic free system
- pH optimum: 7.25- 7.75
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 93
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 6
Illegal BamHI site found at 1044
Illegal XhoI site found at 216 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 714
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 22
Illegal BsaI.rc site found at 1051
References
https://www.sciencedirect.com/science/article/pii/S016816561400193X?via%3Dihub