Difference between revisions of "Part:BBa K2587009"
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<partinfo>BBa_K2587009 short</partinfo>
 
<partinfo>BBa_K2587009 short</partinfo>
  
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ptxD, from <i>Pseudomonas stutzeri</i> is coding for the phosphonate dehydrogenase, that catalyses the oxidation of phosphite (phosphonate) using NAD+ and H20 to Phosphate and NADH. pH optimum is pH = 7.25-7.75.
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<p>Most of the microorganisms, especially common used model organsims like <i>Escherichia coli</i> or <i>Saccharomyces cerevisiae</i> grow only very slow on phosphite as phosphorus source. Moreover in industry is not widely accepted to use antibiotic resistances as markers. Therefore a system is required, which allows avoidance of contamination by other microorganisms and at the same time represents a reliable selection marker. We present the use of the ptxD gene from <i>Pseudomonas stutzeri</i> together with a phosphite media, which could abolish the use of antibiotics in the future. </p>
  
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<h4><strong>Usage and Biology</strong></h4>
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<br>
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<p>Most of the microorganisms, especially common used model organsims like <i>Escherichia coli</i> or <i>Saccharomyces cerevisiae</i> grow only very slow on phosphite as phosphorus source. As a result the ptxD gene together with a phosphite media could be used as a selection marker. Without a permanent usage of antibiotics a screening and a decrease of the contamination risk could be achieved. </p>
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<strong><h4>Usage and Biology</h4></strong>
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<p><ul>
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<li> <strong>ptxD codes for phosphonate dehydrogenase</strong></li>
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<li> <strong>oxidation of phosphite (phosphonate) using NAD+ and H20 to phosphate and NADH </strong></li>
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<li> <strong>Selection marker for budding and fission yeast</strong></li>
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<li> <strong>phosphite-oxidizing ability </strong></li>
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<li> <strong>environmentally safe culture</strong></li>
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<li> <strong>antibiotic free system</strong></li>
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<li> <strong> pH optimum: 7.25- 7.75</strong></li>
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</ul>
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</p>
  
 
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<partinfo>BBa_K2587009 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2587009 SequenceAndFeatures</partinfo>
  
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<h4><strong>References</strong></h4>
  
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<!-- Uncomment this to enable Functional Parameter display
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https://www.sciencedirect.com/science/article/pii/S016816561400193X?via%3Dihub
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===Functional Parameters===
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<partinfo>BBa_K2587009 parameters</partinfo>
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Revision as of 15:36, 9 October 2018


ptxD_opt

Most of the microorganisms, especially common used model organsims like Escherichia coli or Saccharomyces cerevisiae grow only very slow on phosphite as phosphorus source. Moreover in industry is not widely accepted to use antibiotic resistances as markers. Therefore a system is required, which allows avoidance of contamination by other microorganisms and at the same time represents a reliable selection marker. We present the use of the ptxD gene from Pseudomonas stutzeri together with a phosphite media, which could abolish the use of antibiotics in the future.


Usage and Biology

  • ptxD codes for phosphonate dehydrogenase
  • oxidation of phosphite (phosphonate) using NAD+ and H20 to phosphate and NADH
  • Selection marker for budding and fission yeast
  • phosphite-oxidizing ability
  • environmentally safe culture
  • antibiotic free system
  • pH optimum: 7.25- 7.75

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 93
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 6
    Illegal BamHI site found at 1044
    Illegal XhoI site found at 216
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 714
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 22
    Illegal BsaI.rc site found at 1051

References

https://www.sciencedirect.com/science/article/pii/S016816561400193X?via%3Dihub