Difference between revisions of "Part:BBa K2549016"
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====SynNotch receptors function well in Lim WA et al in 2016====
 
====SynNotch receptors function well in Lim WA et al in 2016====
  
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[[File:SynNotch.jpeg|none|360px|thumb|Lim WA et al stated that SynNotch receptors provide extraordinary flexibility in engineering cells with customized sensing/response behaviors to user-specified extracellular cues.]]  
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[[File:SynNotch.jpeg|none|360px|thumb|Lim WA et al stated:''SynNotch receptors provide extraordinary flexibility in engineering cells with customized sensing/response behaviors to user-specified extracellular cues.'']]  
  
 
====LaG17-mN1c-tTAA works as expected====
 
====LaG17-mN1c-tTAA works as expected====

Revision as of 14:10, 9 October 2018


LaG17-mN1c-tTAA

This part is one of our SynNotch receptors[1]. LaG17 (Part:BBa_K2549004) is used as the extracellular sensor module to receive the signal input from GFP. mN1c (Part:BBa_K2549006) is served as the transmembrane core domain of SynNotch, which is evident to have a low basal expression and a high activation efficiency. tTAA (Part:BBa_K2446057) is an improved tetracycline-controlled transcription activator, which is cleaved after SynNotch activation and drives the expression of the amplifier. Besides, a CD8α signal peptide (Part:BBa_K2549044) and a Myc-tag (Part:BBa_K823036) are added to the N terminal of LaG17(Part:BBa_K2549004) for membrane targeting and easy determination of surface expression.

Biology

SynNotch receptors function well in Lim WA et al in 2016

Lim WA et al stated:SynNotch receptors provide extraordinary flexibility in engineering cells with customized sensing/response behaviors to user-specified extracellular cues.

LaG17-mN1c-tTAA works as expected

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 87
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2154
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 127
    Illegal SapI.rc site found at 1069


References

  1. ↑ Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, ..., Thomson M, Lim WA. Cell, 2016 Feb;164(4):780-91 PMID: 26830878; DOI: 10.1016/j.cell.2016.01.012