Difference between revisions of "Part:BBa K2871000"

Revision as of 14:05, 9 October 2018

Map20, T3SS export signal peptide from Map gene

Map20 is a signal sequence encoding a 20 amino acid signal peptide derived from the map gene from the Enteropathogenic E. coli strain E22. It is used as an N-terminal tag for a protein to be translocated by the E. coli type-3-secretion system (T3SS). This T3SS signal tag is described by Charpentier & Oswald (2004) to be sufficient to target proteins to E. coli type III secretion pathway. Its short length seemed to be attractive to us because we wanted to minimize folding interference between the signal sequence and the intended protein fused to the signal sequence.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 85
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K2871000 short</partinfo>
-Map20 is a signal sequence incoding a 20 amino acid signal peptide derived from the map gene from the Enteropathogenic E. coli strain E22. It signals for a protein to be translocated out of the cell by the type-3-secretion system (T3SS). The signal is recognised at N-terminal of the tagged protein.
+Map20 is a signal sequence encoding a 20 amino acid signal peptide derived from the map gene from the Enteropathogenic E. coli strain E22.
+It is used as an N-terminal tag for a protein to be translocated by the  E. coli type-3-secretion system (T3SS). This T3SS signal tag is described by Charpentier & Oswald (2004) to be sufficient to target proteins to E. coli type III secretion pathway. Its short length seemed to be attractive to us because we wanted to minimize folding interference between the signal sequence and the intended protein fused to the signal sequence.
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