Difference between revisions of "Part:BBa K2871006"
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<partinfo>BBa_K2871006 short</partinfo> | <partinfo>BBa_K2871006 short</partinfo> | ||
| − | This part contains two translational units of CesF and CesT E. coli type III secretion system effector chaperone genes. Both CesF (BBa_K2871003) and CesT (BBa_K2871004) CDS are preceded by medium strength RBS, BBa_B0032. 6X Histidine tag is added to the C-terminal of each chaperone gene. | + | This part contains two translational units of CesF and CesT E. coli type III secretion system effector chaperone genes. Both CesF (BBa_K2871003) and CesT (BBa_K2871004) CDS are preceded by weak-medium strength RBS, BBa_B0032. 6X Histidine tag is added to the C-terminal of each chaperone gene to facilitate expression-tracing in western blot. |
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Revision as of 13:29, 9 October 2018
Cassette containing CesF and CesT T3SS substrate chaperones
This part contains two translational units of CesF and CesT E. coli type III secretion system effector chaperone genes. Both CesF (BBa_K2871003) and CesT (BBa_K2871004) CDS are preceded by weak-medium strength RBS, BBa_B0032. 6X Histidine tag is added to the C-terminal of each chaperone gene to facilitate expression-tracing in western blot.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 40
Illegal BglII site found at 545 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 71