Difference between revisions of "Part:BBa K2871006"
Line 3: Line 3:
 
<partinfo>BBa_K2871006 short</partinfo>
 
<partinfo>BBa_K2871006 short</partinfo>
  
−
CesF-CesT chaperone cassette for E. coli T3SS
+
This part contains two translational units of CesF and CesT E. coli type III secretion system effector chaperone genes. Both CesF (BBa_K2871003) and CesT (BBa_K2871004) CDS are preceded by medium strength RBS, BBa_B0032. 6X Histidine tag is added to the C-terminal of each chaperone gene.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 13:26, 9 October 2018


Cassette containing CesF and CesT T3SS substrate chaperones

This part contains two translational units of CesF and CesT E. coli type III secretion system effector chaperone genes. Both CesF (BBa_K2871003) and CesT (BBa_K2871004) CDS are preceded by medium strength RBS, BBa_B0032. 6X Histidine tag is added to the C-terminal of each chaperone gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 40
    Illegal BglII site found at 545
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 71