Difference between revisions of "Part:BBa K2610008"
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MreB, a homologue of actin, is a protein which is expressed in nearly all rod-shaped bacterial cells. It is one of the three types of actin-like filaments present in bacteria. MreB is important for maintenance of cell width and cell viability as it recruits the machinery responsible for the establishment of peptidoglycan, an essential building block for the cell wall. | MreB, a homologue of actin, is a protein which is expressed in nearly all rod-shaped bacterial cells. It is one of the three types of actin-like filaments present in bacteria. MreB is important for maintenance of cell width and cell viability as it recruits the machinery responsible for the establishment of peptidoglycan, an essential building block for the cell wall. | ||
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| + | ===Usage and Biology=== | ||
| + | <p>iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) using flow cytometry. | ||
| + | We fused promoters to green fluorescent protein and stressed transformed DH5a cells for several hours. </p> <br> | ||
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| + | [[File:T--Leiden--heatmap.png|750px]] <br> | ||
| + | <span style="font-size:1em"><b>Figure 1.</b> Heatmap with stress-activated promoters </span> | ||
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| + | As can be observed in Figure 1 the MreB promoter is only moderately activated by nalidixic acid and tetracycline | ||
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Revision as of 13:06, 9 October 2018
pMreB
MreB, a homologue of actin, is a protein which is expressed in nearly all rod-shaped bacterial cells. It is one of the three types of actin-like filaments present in bacteria. MreB is important for maintenance of cell width and cell viability as it recruits the machinery responsible for the establishment of peptidoglycan, an essential building block for the cell wall.
Usage and Biology
iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) using flow cytometry. We fused promoters to green fluorescent protein and stressed transformed DH5a cells for several hours.
Figure 1. Heatmap with stress-activated promoters
As can be observed in Figure 1 the MreB promoter is only moderately activated by nalidixic acid and tetracycline
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]