Difference between revisions of "Part:BBa K2610002"
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<partinfo>BBa_K2610002 short</partinfo> | <partinfo>BBa_K2610002 short</partinfo> | ||
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| + | <p>Small heat shock proteins IbpA and IbpB are involved in heat shock response and oxidative stress. The promoter of IbpA has previously been defined as a possible stress probe in the heat shock response of E.coli. </p><br> | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | <p | + | <p>iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated against a repertoire of known antibiotics (Figure 1) </p> <br> |
[[File:T--Leiden--heatmap.png|750px]] <br> | [[File:T--Leiden--heatmap.png|750px]] <br> | ||
Revision as of 12:22, 9 October 2018
pIbpA
Small heat shock proteins IbpA and IbpB are involved in heat shock response and oxidative stress. The promoter of IbpA has previously been defined as a possible stress probe in the heat shock response of E.coli.
Usage and Biology
iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated against a repertoire of known antibiotics (Figure 1)
Figure 1. Heatmap with stress-activated promoters
As can be observed in Figure 1 the ibpA promoter is only moderately activated by streptomycin and tetracycline.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]