Difference between revisions of "Part:BBa K2797013"
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pSB1C3 with a constituitively expressed RFP construct under the control of a medium strength Anderson promoter. | pSB1C3 with a constituitively expressed RFP construct under the control of a medium strength Anderson promoter. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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In summer 2018, the Newcastle University iGEM team designed a constitutive, medium strength RFP expression construct and cloned said construct into the backbone of the iGEM InterLab test device pSB1C3 vectors - in the non-coding region between the chloramphenicol resistance gene and the Origin of Replication - for use as an Internal Standard within the iGEM 2018 InterLab study. | In summer 2018, the Newcastle University iGEM team designed a constitutive, medium strength RFP expression construct and cloned said construct into the backbone of the iGEM InterLab test device pSB1C3 vectors - in the non-coding region between the chloramphenicol resistance gene and the Origin of Replication - for use as an Internal Standard within the iGEM 2018 InterLab study. | ||
Revision as of 11:32, 9 October 2018
High copy BioBrick assembly plasmid pSB1C3 with RFP internal standard
pSB1C3 with a constituitively expressed RFP construct under the control of a medium strength Anderson promoter.
Usage and Biology
In summer 2018, the Newcastle University iGEM team designed a constitutive, medium strength RFP expression construct and cloned said construct into the backbone of the iGEM InterLab test device pSB1C3 vectors - in the non-coding region between the chloramphenicol resistance gene and the Origin of Replication - for use as an Internal Standard within the iGEM 2018 InterLab study.
The addition of this RFP Internal Standard within the InterLab study devices revealed that, even under the same experimental conditions, the complex nature of biological systems still cause variation in protein expression, and highlights how important the use of an Internal Standard is in the identification of variation in part characterisation studies.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 989
Illegal suffix found in sequence at 1
Illegal EcoRI site found at 3052
Illegal XbaI site found at 3067
Illegal SpeI site found at 1905
Illegal PstI site found at 1919 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 989
Illegal EcoRI site found at 3052
Illegal NheI site found at 1017
Illegal NheI site found at 1040
Illegal SpeI site found at 2
Illegal SpeI site found at 1905
Illegal PstI site found at 16
Illegal PstI site found at 1919
Illegal NotI site found at 9
Illegal NotI site found at 995
Illegal NotI site found at 1912
Illegal NotI site found at 3058 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 989
Illegal EcoRI site found at 3052
Illegal XhoI site found at 2036
Illegal XhoI site found at 2928 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 989
Illegal suffix found in sequence at 2
Illegal EcoRI site found at 3052
Illegal XbaI site found at 3067
Illegal SpeI site found at 1905
Illegal PstI site found at 1919 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 989
Illegal EcoRI site found at 3052
Illegal XbaI site found at 1004
Illegal XbaI site found at 3067
Illegal SpeI site found at 2
Illegal SpeI site found at 1905
Illegal PstI site found at 16
Illegal PstI site found at 1919
Illegal AgeI site found at 1627
Illegal AgeI site found at 1739 - 1000COMPATIBLE WITH RFC[1000]