Difference between revisions of "Part:BBa K2610002"
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Small heat shock proteins IbpA and IbpB are involved in heat shock response and oxidative stress. The promoter of IbpA has previously been defined as a possible stress probe in the heat shock response of E.coli. | Small heat shock proteins IbpA and IbpB are involved in heat shock response and oxidative stress. The promoter of IbpA has previously been defined as a possible stress probe in the heat shock response of E.coli. | ||
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iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated against a repertoire of known antibiotics (Figure 1) | iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated against a repertoire of known antibiotics (Figure 1) | ||
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As can be observed in Figure 1 the ibpA promoter is only moderately activated by streptomycin and tetracycline. | As can be observed in Figure 1 the ibpA promoter is only moderately activated by streptomycin and tetracycline. | ||
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| + | <!-- Add more about the biology of this part here | ||
| + | ===Usage and Biology=== | ||
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Revision as of 10:57, 9 October 2018
pIbpA
Small heat shock proteins IbpA and IbpB are involved in heat shock response and oxidative stress. The promoter of IbpA has previously been defined as a possible stress probe in the heat shock response of E.coli.
iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated against a repertoire of known antibiotics (Figure 1)
<img src="
" max-width="30%">
Figure 1. Heatmap with stress-activated promoters
As can be observed in Figure 1 the ibpA promoter is only moderately activated by streptomycin and tetracycline.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]