Difference between revisions of "Part:BBa K2889003:Experience"
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===Applications of BBa_K2889003=== | ===Applications of BBa_K2889003=== | ||
| + | In our previous project, we constructed two miR-21 sponge plasmid, pEGFP-miR-21-sponge-2s and pEGFP-miR-21-sponge-6s, as the monitor to detect the expression of miR-21 in cells.However, we’d like to ascertain a preferable binding sites number contained in miRNA sponge as a sensitivity and specificity monitor this year.Hence, we constructed pEGFP-miR-21-sponge-4s.We find that miR-21-sponge-4s is more sensitive than miR-21-sponge-2s and miR-21-sponge6s. | ||
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| + | https://static.igem.org/mediawiki/parts/0/01/The_effect_of_pEGFP-miR-21-sponge-4s_on_the_correlative_between_fluorescence_value_of_GFP_and_copy_numbers_of_miR-21.jpg | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 06:35, 9 October 2018
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2889003
In our previous project, we constructed two miR-21 sponge plasmid, pEGFP-miR-21-sponge-2s and pEGFP-miR-21-sponge-6s, as the monitor to detect the expression of miR-21 in cells.However, we’d like to ascertain a preferable binding sites number contained in miRNA sponge as a sensitivity and specificity monitor this year.Hence, we constructed pEGFP-miR-21-sponge-4s.We find that miR-21-sponge-4s is more sensitive than miR-21-sponge-2s and miR-21-sponge6s.
User Reviews
UNIQb84e910e29e396a4-partinfo-00000000-QINU UNIQb84e910e29e396a4-partinfo-00000001-QINU