Difference between revisions of "Part:BBa K2631000"
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| − | ADTZ, which is also called aflatoxin oxidase (AFO), is the first enzyme identified to bed able to degrade AFB1. It can be isolated from intracellular extracts and show a strong affinity with AFB1. As a potential degradation enzyme candidate in our project. | + | ADTZ, which is also called aflatoxin oxidase (AFO), is the first enzyme identified to bed able to degrade AFB1. It can be isolated from intracellular extracts and show a strong affinity with AFB1. As a potential degradation enzyme candidate in our project. In order to express soluble ADTZ in E．coli，the DNA coding ADTZ was inserted into the vector pMAL-c5x to produce the fusion protein of maltose-binding protein (MBP) and ADTZ. |
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Revision as of 05:47, 9 October 2018
ADTZ Enzyme
ADTZ, which is also called aflatoxin oxidase (AFO), is the first enzyme identified to bed able to degrade AFB1. It can be isolated from intracellular extracts and show a strong affinity with AFB1. As a potential degradation enzyme candidate in our project. In order to express soluble ADTZ in E．coli，the DNA coding ADTZ was inserted into the vector pMAL-c5x to produce the fusion protein of maltose-binding protein (MBP) and ADTZ.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1556
Illegal BglII site found at 1979 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]