Difference between revisions of "Part:BBa K2889003:Experience"
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We digested the PSB1C3 vectors with restriction enzymes EcoRI and PstI | We digested the PSB1C3 vectors with restriction enzymes EcoRI and PstI | ||
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| + | We selected the positive clones by PCR and sequencing | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 04:43, 9 October 2018
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2889003
First we amplified miR-21-sponge-4s using primers. After that, we purified the PCR products by PCR Purification Kit and digested them with restriction enzymes EcoR I and Pst I
We digested the PSB1C3 vectors with restriction enzymes EcoRI and PstI
We selected the positive clones by PCR and sequencing
User Reviews
UNIQbda12792611259c8-partinfo-00000000-QINU UNIQbda12792611259c8-partinfo-00000001-QINU