Difference between revisions of "Part:BBa K2665013"

Revision as of 01:52, 9 October 2018 (view source) TongYou (Talk | contribs) ← Older edit		Revision as of 02:45, 9 October 2018 (view source) TongYou (Talk | contribs) Newer edit →
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	<span class='h3bb'>Sequence and Features</span>		<span class='h3bb'>Sequence and Features</span>
	<partinfo>BBa_K2665013 SequenceAndFeatures</partinfo>		<partinfo>BBa_K2665013 SequenceAndFeatures</partinfo>
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		+	==Reference==
		+	'''[https://parts.igem.org/Part:BBa_K2225000 BBa_K2225000]'''
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		+	<!-- -->
		+	<span class='h3bb'>Sequence and Features</span>
		+	<partinfo>BBa_K2403003 SequenceAndFeatures</partinfo>
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Revision as of 02:45, 9 October 2018

TDH3-AtNHXS1-6xHis-CYC

AtNHXS1 is a Na+/H+ antiporter which is located in the membrane of vacuoles and that original comes from Arabidopsis thaliana.

We assembled BBa_K2225000 with TDpromoter and CYC1 terminator to express the protein, and we added His-tag in case of Western blotting.

Usage and Biology

This part sequence can be used directly because it contains promoter and terminator, but promoter is TDH3 promoter so it can't express in E. coli. AtNHXS1 is a Na+/H+ antiporter which is located in the membrane of vacuoles, so we used this part in order to increase the amount of Na+ uptake of yeast.

Characterization

Sequence and Features

Reference

BBa_K2225000

Sequence and Features