Difference between revisions of "Part:BBa K2665012"

Revision as of 02:31, 9 October 2018

TDH3-ZrGPD1-6xHis-CYC

ZrGPD1 is the gene that codes a glycerol-3-phosphate dehydrogenase which controls glycerol synthesis in Zygosaccharomyces rouxii. It increases glycerol synthesis which work as compatible solute. So, it may raise osmotolerance in yeast.

Usage and Biology

This part sequence can be used directly because it contains promoter and terminator, but promoter is TDH3 promoter so it can't express in E. coli. If need to use the protein in E. coli, please note that you have to change promoter and terminator. ZrGPD1 is the gene that codes a glycerol-3-phosphate dehydrogenase which controls glycerol synthesis.

Characterization

We used this part in order to enhance the solt tolerance of yeast. We assessed the tolerance in our method(please refer to [http://http://2018.igem.org/Team:Kyoto/Protocol our Wiki's Special Protocol] ).

Result is showed below.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1923
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1454
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1129
Illegal BsaI site found at 1889
Illegal BsaI.rc site found at 1271

Reference

[1] Hou,Lihua Wang,Meng Wang,Cong Wang,Chunling Wang,Haiyong (2013) Analysis of salt-tolerance genes in zygosaccharomyces rouxii, Applied Biochemistry and Biotechnoloogy 1417-1425

@@ Line 15: / Line 15: @@
 We used this part in order to enhance the solt tolerance of yeast.
 We assessed the tolerance in our method(please refer to '''[http://http://2018.igem.org/Team:Kyoto/Protocol our Wiki's Special Protocol]''' ).
 Result is showed below.