Difference between revisions of "Part:BBa K2665013"

Revision as of 01:52, 9 October 2018

TDH3-AtNHXS1-6xHis-CYC

AtNHXS1 is a Na+/H+ antiporter which is located in the membrane of vacuoles and that original comes from Arabidopsis thaliana.

We assembled BBa_K2225000 with TDpromoter and CYC1 terminator to express the protein, and we added His-tag in case of Western blotting.

Usage and Biology

This part sequence can be used directly because it contains promoter and terminator, but promoter is TDH3 promoter so it can't express in E. coli. AtNHXS1 is a Na+/H+ antiporter which is located in the membrane of vacuoles, so we used this part in order to increase the amount of Na+ uptake of yeast.

Characterization

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1446
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 AtNHXS1 is a Na+/H+ antiporter which is located in the membrane of vacuoles and that original comes from Arabidopsis thaliana.
 We assembled '''[https://parts.igem.org/Part:BBa_K2225000 BBa_K2225000]''' with TDpromoter and CYC1 terminator to express the protein, and we added His-tag in case of Western blotting.
-<!-- Add more about the biology of this part here
+<!-- Add more about the biology of this part here-->
 ==Usage and Biology==
 This part sequence can be used directly because it contains promoter and terminator, but promoter is TDH3 promoter so it can't express in <i>E. coli</i>. AtNHXS1 is a Na+/H+ antiporter which is located in the membrane of vacuoles, so we used this part in order to increase the amount of Na+ uptake of yeast.