Difference between revisions of "Part:BBa K2683001:Design"

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===Design Notes===
 
===Design Notes===
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Codon optimization was ideal for characterization and application by using E.coli.  
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Although originally codon optimized for E.coli, sequencing issues lead us to working with genomic DNA.
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===Source===
 
===Source===

Revision as of 22:36, 8 October 2018


Arc Protein Codon optimized for E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 531
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Although originally codon optimized for E.coli, sequencing issues lead us to working with genomic DNA.

Source

The original sequence is derived from the Arc protein found in mouse neural cells. The DNA was amplified from the mouse genome and made biobrick compatable by using primer extension PCR and site directed mutagenesis.

References