Difference between revisions of "Part:BBa K2686000"

Revision as of 14:52, 8 October 2018

Encapsulin with HexaHistidine insert and C-terminal OT1

This part codes a modified Thermotoga maritima Encapsulin protein. The part is optimized for expression in E. coli and has an additional HexaHistidine insert between amino acids 43 and 44, forming a loop on the interior surface of the encapsulin monomer providing higher heat resistance and stability (Moon et al., 2014). The C-terminus of the encapsulin is fused to a SIINFEKL (OVA) peptide which is displayed on the exterior surface of the encapsulin momomer as an antigen (Choi et al., 2016).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 77
Illegal BglII site found at 492
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 426
Illegal SapI.rc site found at 457

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2686000 short</partinfo>
-This part codes a modified Thermotoga maritima Encapsulin protein. The part is optimized for expression in E. coli and has an additional HexaHistidine insert between amino acids 43 and 44, forming a loop on the interior surface of the encapsulin monomer providing higher heat resistance and stability. The C-terminus of the encapsulin is fused to a SIINFEKL (OVA) peptide which is displayed on the exterior surface of the encapsulin momomer as an antigen.
+This part codes a modified Thermotoga maritima Encapsulin protein. The part is optimized for expression in E. coli and has an additional HexaHistidine insert between amino acids 43 and 44, forming a loop on the interior surface of the encapsulin monomer providing higher heat resistance and stability (Moon et al., 2014). The C-terminus of the encapsulin is fused to a SIINFEKL (OVA) peptide which is displayed on the exterior surface of the encapsulin momomer as an antigen (Choi et al., 2016).
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