Difference between revisions of "Part:BBa K2686002"
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<partinfo>BBa_K2686002 short</partinfo>
 
<partinfo>BBa_K2686002 short</partinfo>
  
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This is a BioBrick containing the sequence for Thermotoga maritima encapsulin, a bacterial protein nanocompartment which self assembles to form a 60-mer. The part can be used to deliver cargo, both on the outer surface of the nanoparticle by fusing a peptide in between the 139/140 Amino Acids as well as the protein's C terminus, or alternately by attaching drugs using 1,4 Michael additions (Thiol -Maleimide reactions) to a cysteine present on the surface of each subunit. Cargo proteins can also be loaded inside the nanocage using a tag binding to Encapsulin's interior surface. The protein is modified with an additonal amino acid sequence (GGGGGGHHHHHHGGGGG) between positions 43/44 granting it better stability and high heat resistance.  
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This is a BioBrick containing the sequence for Thermotoga maritima encapsulin, a bacterial protein nanocompartment which self assembles to form a 60-mer. The part can be used to deliver cargo, both on the outer surface of the nanoparticle by fusing a peptide in between the 139/140 Amino Acids as well as the protein's C terminus. Cargo proteins can also be loaded inside the nano-cage using a tag binding to Encapsulin's interior surface. The protein is modified with an additonal amino acid sequence (GGGGGGHHHHHHGGGGG) between positions 43/44 granting it better stability and high heat resistance [1].
  
 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K2686002 parameters</partinfo>
 
<partinfo>BBa_K2686002 parameters</partinfo>
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<!-- References -->
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[1] Developing Genetically Engineered Encapsulin Protein Cage Nanoparticles as a Targeted Delivery Nanoplatform
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Hyojin Moon, Jisu Lee, Junseon Min, and Sebyung Kang
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Biomacromolecules 2014 15 (10), 3794-3801
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DOI: 10.1021/bm501066m

Revision as of 14:07, 8 October 2018


Encapsulin protein with HexaHistidine insert

This is a BioBrick containing the sequence for Thermotoga maritima encapsulin, a bacterial protein nanocompartment which self assembles to form a 60-mer. The part can be used to deliver cargo, both on the outer surface of the nanoparticle by fusing a peptide in between the 139/140 Amino Acids as well as the protein's C terminus. Cargo proteins can also be loaded inside the nano-cage using a tag binding to Encapsulin's interior surface. The protein is modified with an additonal amino acid sequence (GGGGGGHHHHHHGGGGG) between positions 43/44 granting it better stability and high heat resistance [1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 77
    Illegal BglII site found at 492
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 426
    Illegal SapI.rc site found at 457


[1] Developing Genetically Engineered Encapsulin Protein Cage Nanoparticles as a Targeted Delivery Nanoplatform Hyojin Moon, Jisu Lee, Junseon Min, and Sebyung Kang Biomacromolecules 2014 15 (10), 3794-3801 DOI: 10.1021/bm501066m