Difference between revisions of "Part:BBa K2541201:Design"

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===Design Notes===
 
===Design Notes===
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When designing the sequence, you should consider its pairing with other bases, which may affect its free energy.
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The secondary structure is important to the function of an RNA thermosensor. We designed the thermosensor according to the stem length,loop size and base bulge.
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===Source===
 
===Source===

Revision as of 05:19, 8 October 2018


Cold-repressible RNA-based thermosensor-1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The secondary structure is important to the function of an RNA thermosensor. We designed the thermosensor according to the stem length,loop size and base bulge.

Source

E.coli

References

Pertzev A V, Nicholson A W. Characterization of RNA sequence determinants and antideterminants of processing reactivity for a minimal substrate of Escherichia coli ribonuclease III[J]. Nucleic Acids Research, 2006, 34(13):3708-3721.