Difference between revisions of "Part:BBa K2889000"

 
 
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<partinfo>BBa_K2889000 short</partinfo>
 
<partinfo>BBa_K2889000 short</partinfo>
  
We cloned the full length of IL7-AS, two truncated sequences of IL7-AS (IL7-AS-S1 and IL7-AS-S2) into PCDNA3.1 and transfected these plasmids to 786-O cells. Through in vitro scratch wound healing assay, overexpression of IL7-AS promoted cell migration of 786-O cells. However IL7-AS-S1 and IL7-AS-S2 did not influence the migration of 786-O cells, suggesting IL7-AS-S1 and IL7-AS-S2 lack of key structural domains.
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IL7-AS [located anti-sense to interleukin-7 (Il7) gene; Accession number: NM_000880.3] is a newly discovered lncRNA that can be induced across multiple human and mouse cell types and has been reported to regulate the expression of interleukin-6 (IL6).  IL6 is emerging as a major regulator of renal cell cancer. However, roles of IL7-AS in renal cell cancer and the related molecular mechanisms remain largely undetermined.We cloned the truncated sequences of IL7-AS (IL7-AS-S2 contain 411bp) into pSB1C3 for submitting. And we also cloned IL7-AS-S2 into PCDNA3.1 and transfected these plasmids into 786-O cells. Through in vitro scratch wound healing assay, overexpression of IL7-AS-S2 promoted cell migration of 786-O cells. These results suggested that IL7-AS-S2 contain key structural domains.
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Latest revision as of 02:56, 8 October 2018


pSB1C3-IL7-AS-S2

IL7-AS [located anti-sense to interleukin-7 (Il7) gene; Accession number: NM_000880.3] is a newly discovered lncRNA that can be induced across multiple human and mouse cell types and has been reported to regulate the expression of interleukin-6 (IL6). IL6 is emerging as a major regulator of renal cell cancer. However, roles of IL7-AS in renal cell cancer and the related molecular mechanisms remain largely undetermined.We cloned the truncated sequences of IL7-AS (IL7-AS-S2 contain 411bp) into pSB1C3 for submitting. And we also cloned IL7-AS-S2 into PCDNA3.1 and transfected these plasmids into 786-O cells. Through in vitro scratch wound healing assay, overexpression of IL7-AS-S2 promoted cell migration of 786-O cells. These results suggested that IL7-AS-S2 contain key structural domains.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 198
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]