Difference between revisions of "Part:BBa K2889002"

Latest revision as of 02:41, 8 October 2018

pSB1C3-IL7-AS-S1

We cloned the truncated sequences of IL7-AS (IL7-AS-S1) into PCDNA3.1 and transfected these plasmids into 786-O cells. Through in vitro scratch wound healing assay, overexpression of IL7-AS-S1 promoted cell migration of 786-O cells. These results suggested that IL7-AS-S1 contain key structural domains.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 600
Illegal SpeI site found at 813
Illegal SpeI site found at 924
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 626
Illegal SpeI site found at 813
Illegal SpeI site found at 924
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 600
Illegal SpeI site found at 813
Illegal SpeI site found at 924
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 600
Illegal SpeI site found at 813
Illegal SpeI site found at 924
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2889002 short</partinfo>
-We cloned the full length of IL7-AS, two truncated sequences of IL7-AS (IL7-AS-S1 and IL7-AS-S2) into PCDNA3.1 and transfected these plasmids to 786-O cells. Through in vitro scratch wound healing assay, overexpression of IL7-AS promoted cell migration of 786-O cells. However IL7-AS-S1 and IL7-AS-S2 did not influence the migration of 786-O cells, suggesting IL7-AS-S1 and IL7-AS-S2 lack of key structural domains.
+We cloned the truncated sequences of IL7-AS (IL7-AS-S1) into PCDNA3.1 and transfected these plasmids into 786-O cells. Through in vitro scratch wound healing assay, overexpression of IL7-AS-S1 promoted cell migration of 786-O cells. These results suggested that IL7-AS-S1 contain key structural domains.
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