Difference between revisions of "Part:BBa K2573000"
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| − | A 2.4kb fragment containing the MetE gene from plasmid pSK397 was cloned into bba_J4450 via PCR and NEBuild Hi-Fi Assembly. | + | This part is the MetE gene cassette under the inducible LacI promoter. A 2.4kb fragment containing the MetE gene from plasmid pSK397 was cloned into bba_J4450 via PCR and NEBuild Hi-Fi Assembly. |
Revision as of 00:03, 8 October 2018
MetE Coding Device
Usage and Biology
Methionine is an essential amino acid for E.coli growth. E. coli MetE codes for an enzyme that catalyzes the final step of de novo methionine biosynthesis without using an intermediate methyl carrier and in the absence of vitamin B12.
This part is the MetE gene cassette under the inducible LacI promoter. A 2.4kb fragment containing the MetE gene from plasmid pSK397 was cloned into bba_J4450 via PCR and NEBuild Hi-Fi Assembly.
REFERENCES:
Pejchal, Robert, and Martha L Ludwig. “Cobalamin-Independent Methionine Synthase (MetE): A Face-to-Face Double Barrel That Evolved by Gene Duplication.” PLOS ONE, Public Library of Science, journals.plos.org/plosbiology/article?id=10.1371%2Fjournal.pbio.0030031.
“Escherichia Coli K-12 Substr. MG1655 MetE.” MetaCyc Parathion Hydrolase, biocyc.org/gene?orgid=ECOLI&id=EG10584.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1864
- 1000COMPATIBLE WITH RFC[1000]