Difference between revisions of "Part:BBa K2609026"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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<h2> Biology </h2> | <h2> Biology </h2> | ||
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We used the this Lambda RED recombination system with a T4 Endolysin gene (<a href="https://parts.igem.org/Part:BBa_K2609017">BBa_K2609017</a>) to recombine Bacteriophage T4 and create a lysis deficient phage. This lysis deficient phage triggers its host to produce a monocyte chemoattractant <i>mcp-1</i> (<a href="https://parts.igem.org/Part:BBa_K2609000">BBa_K2609000</a>). | We used the this Lambda RED recombination system with a T4 Endolysin gene (<a href="https://parts.igem.org/Part:BBa_K2609017">BBa_K2609017</a>) to recombine Bacteriophage T4 and create a lysis deficient phage. This lysis deficient phage triggers its host to produce a monocyte chemoattractant <i>mcp-1</i> (<a href="https://parts.igem.org/Part:BBa_K2609000">BBa_K2609000</a>). | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 21:38, 7 October 2018
Lambda Red Recombinases under T7 expression system
This part generates the three Lambda ReD recombinases - exo, gam, beta - when expressed in a T7 expression strain like E. coli BL21(DE3).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1943
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1460
- 1000COMPATIBLE WITH RFC[1000]