Difference between revisions of "Part:BBa K2871002"

Latest revision as of 18:37, 7 October 2018

mCherry, N-terminally fused with Map20 signal for T3SS export

The mCherry fluorescent protein from BBa_J06504 fused with the polypeptide signal sequence map20 (BBa_K2871000), at the N-terminal. This enables mCherry to be translocated out of the cell via the bacterial Type-3-secretion system (T3SS). the signal sequence is derived from the map gene from the Enteropathogenic E. coli strain E22.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2871002 short</partinfo>
-The mCherry fluorescent protein from BBa_J06504 fused with the polypeptide signal sequence map20 (BBa_K2871002), at the N-terminal. This enables mCherry to be translocated out of the cell via the bacterial Type-3-secretion system (T3SS). the signal sequence is derived from the map gene from the Enteropathogenic E. coli strain E22.
+The mCherry fluorescent protein from BBa_J06504 fused with the polypeptide signal sequence map20 (BBa_K2871000), at the N-terminal. This enables mCherry to be translocated out of the cell via the bacterial Type-3-secretion system (T3SS). the signal sequence is derived from the map gene from the Enteropathogenic E. coli strain E22.
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