Difference between revisions of "Part:BBa K2871003:Design"

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https://www.sciencedirect.com/science/article/pii/S1931312807003095
 
https://www.sciencedirect.com/science/article/pii/S1931312807003095
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https://www.researchgate.net/publication/277411140_Engineering_the_Controlled_Assembly_of_Filamentous_Injectisomes_in_E_coli_K-12_for_Protein_Translocation_into_Mammalian_Cells

Latest revision as of 17:56, 7 October 2018


CesF T3SS substrate chaperone


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 21
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 52


Design Notes

The CesF and CesT chaperone gene is present in a genomic region called 'locus of enterocyte effacement' (LEE) of Enteropathogenic E. coli. We found that the original CesF DNA sequence contains EcoRI restriction site so we choose to order codon-optimized synthetic gene from IDT to eliminate most common restriction sites.


Source

Amino acid sequence retrieved from Enteropathogenic E. coli (EPEC) O127:H6 strain E2348/69. Codon-optimized and synthesized by IDT.

References

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC490934

https://www.sciencedirect.com/science/article/pii/S1931312807003095

https://www.researchgate.net/publication/277411140_Engineering_the_Controlled_Assembly_of_Filamentous_Injectisomes_in_E_coli_K-12_for_Protein_Translocation_into_Mammalian_Cells