Difference between revisions of "Part:BBa K2675040:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
BBa_J23102 Promoter + RBS (BBa_K2675010) + AimR-Phi3T coding sequence (BBa_K2675000) + ECK120029600 Terminator (BBa_K2486006)
+
BBa_J23102 Promoter + RBS (BBa_K2675010) + AimR-Phi3T coding sequence (BBa_K2675000) + ECK120029600 Terminator (BBa_K2486006).
−
 
+
−
 
+
  
 
===Source===
 
===Source===
Line 16: Line 14:
  
 
===References===
 
===References===
 +
[1] Erez Z, Steinberger-Levy I, Shamir M, Doron S, Stokar-Avihail A, Peleg Y, Melamed S, Leavitt A, Savidor A, Albeck S, Amitai G, Sorek R. Communication between viruses guides lysis-lysogeny decisions. Nature (2017) 541, 488-493.

Revision as of 17:03, 7 October 2018


AimR of phage phi3T expression cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa_J23102 Promoter + RBS (BBa_K2675010) + AimR-Phi3T coding sequence (BBa_K2675000) + ECK120029600 Terminator (BBa_K2486006).

Source

DNA synthesis

References

[1] Erez Z, Steinberger-Levy I, Shamir M, Doron S, Stokar-Avihail A, Peleg Y, Melamed S, Leavitt A, Savidor A, Albeck S, Amitai G, Sorek R. Communication between viruses guides lysis-lysogeny decisions. Nature (2017) 541, 488-493.