Difference between revisions of "Part:BBa K2669003:Design"

(Design Notes)
(Source)
Line 8: Line 8:
 
===Source===
 
===Source===
  
RBS (BBa_J34801), constitutive promotor (BBa_J23119), optimized AmilCP (BBa_K2669002) and double terminator (BBa_B0014), primer binding sites VF2 and VR (BBa_G00100), (BBa_G00102), prefix and suffix in pbs1c3 backbone.  
+
constitutive promotor (BBa_J23119), RBS (BBa_J34801), codon optimized original native AmilCP (BBa_K2669002) and double terminator (BBa_B0014).
  
 
===References===
 
===References===

Revision as of 16:30, 7 October 2018


Strong constitutive promotor + RBS + Optimized Amil CP (BBa_K2669002) + double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

constitutive promotor (BBa_J23119), RBS (BBa_J34801), codon optimized original native AmilCP (BBa_K2669002) and double terminator (BBa_B0014).

References