Difference between revisions of "Part:BBa K2675017"
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  Using the  Salis Lab RBS calculator v2.0 [1, 2], the predicted features of this RBS are:
 
  Using the  Salis Lab RBS calculator v2.0 [1, 2], the predicted features of this RBS are:
−
  Translation Initiation Rate (au) : 202865,54
+
  Translation Initiation Rate (au) : 18413,48
−
  dG_total (kcal/mol) : -11,34
+
  dG_total (kcal/mol) : -6,01
−
  dG_mRNA_rRNA (kcal/mol) : -20,08
+
  dG_mRNA_rRNA (kcal/mol) : -11,31
 
  dG_spacing (kcal/mol) : 0
 
  dG_spacing (kcal/mol) : 0
−
  dG_standby (kcal/mol) : 0
+
  dG_standby (kcal/mol) : 1,52
 
  dG_start (kcal/mol) : -2,76
 
  dG_start (kcal/mol) : -2,76
−
  dG_mRNA (kcal/mol) : -12,28
+
  dG_mRNA (kcal/mol) : -7,32
−
  Accuracy (warnings) : OK
+
  Accuracy (warnings) : NoEQ
  
  

Revision as of 14:25, 7 October 2018


Synthetic RBS designed for sfGFP

This part is a synthetic RBS designed for sfGFP (BBa_K2675005) and sfGFP-LVAtag (BBa_K2675006) using the Salis Lab RBS calculator v2.0 [1, 2].

Usage and Biology

This RBS was used to drive the expression of sfGFP (BBa_K2675005) and sfGFP-LVAtag (BBa_K2675006) under the control of the BBa_J23110 promoter in the composite parts BBa_K2675052, BBa_K2675062, .... 

Using the  Salis Lab RBS calculator v2.0 [1, 2], the predicted features of this RBS are:
Translation Initiation Rate (au)	:	18413,48
dG_total (kcal/mol)	:	-6,01
dG_mRNA_rRNA (kcal/mol)	:	-11,31
dG_spacing (kcal/mol)	:	0
dG_standby (kcal/mol)	:	1,52
dG_start (kcal/mol)	:	-2,76
dG_mRNA (kcal/mol)	:	-7,32
Accuracy (warnings)	:	NoEQ


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]