Difference between revisions of "Part:BBa K2675011"

Revision as of 14:02, 7 October 2018

Synthetic RBS designed for AimP of Phi3T

This part is a synthetic RBS designed for AimP, the immature arbitrium peptide of phage phi3T (BBa_K2675001) using the Salis Lab RBS calculator v2.0 [1, 2].

Usage and Biology

This RBS was used to drive the expression of AimP (BBa_K2675001) under the control of the BBa_J23100 promoter in the composite part BBa_K2675041.

Using the  Salis Lab RBS calculator v2.0 [1, 2], the predicted features of this RBS are:
Translation Initiation Rate (au)	:	36520,42
dG_total (kcal/mol)	:	-7,53
dG_mRNA_rRNA (kcal/mol)	:	-11,61
dG_spacing (kcal/mol)	:	0
dG_standby (kcal/mol)	:	0
dG_start (kcal/mol)	:	-2,76
dG_mRNA (kcal/mol)	:	-7,62
Accuracy (warnings)	:	OK

Sequence and Features