Difference between revisions of "Part:BBa K2675013"
| Line 3: | Line 3: | ||
<partinfo>BBa_K2675013 short</partinfo> | <partinfo>BBa_K2675013 short</partinfo> | ||
| − | + | This part is a synthetic RBS designed for PelB-SAIRGA ([[Part:BBa_K2675003|BBa_K2675003]]) using the [https://salislab.net/software/forward Salis Lab RBS calculator v2.0] [1, 2]. | |
| − | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | This RBS was used to drive the expression of PelB-SAIRGA ([[Part:BBa_K2675003|BBa_K2675003]]) under the control of the ([[Part:BBa_J23100|BBa_J23100]]) promoter in the composite part ([[Part:BBa_K2675043|BBa_K2675043]]). | ||
| + | |||
| + | Using the Salis Lab RBS calculator v2.0 [1, 2], the predicted features of this RBS are: | ||
| + | Translation Initiation Rate (au) : 413347,83 | ||
| + | dG_total (kcal/mol) : -12,92 | ||
| + | dG_mRNA_rRNA (kcal/mol) : -22,03 | ||
| + | dG_spacing (kcal/mol) : 0 | ||
| + | dG_standby (kcal/mol) : 0 | ||
| + | dG_start (kcal/mol) : -2,76 | ||
| + | dG_mRNA (kcal/mol) : -12,44 | ||
| + | Accuracy (warnings) : NoEQ | ||
<!-- --> | <!-- --> | ||
Revision as of 13:49, 7 October 2018
Synthetic RBS designed for PelB-SAIRGA
This part is a synthetic RBS designed for PelB-SAIRGA (BBa_K2675003) using the Salis Lab RBS calculator v2.0 [1, 2].
Usage and Biology
This RBS was used to drive the expression of PelB-SAIRGA (BBa_K2675003) under the control of the (BBa_J23100) promoter in the composite part (BBa_K2675043).
Using the Salis Lab RBS calculator v2.0 [1, 2], the predicted features of this RBS are: Translation Initiation Rate (au) : 413347,83 dG_total (kcal/mol) : -12,92 dG_mRNA_rRNA (kcal/mol) : -22,03 dG_spacing (kcal/mol) : 0 dG_standby (kcal/mol) : 0 dG_start (kcal/mol) : -2,76 dG_mRNA (kcal/mol) : -12,44 Accuracy (warnings) : NoEQ
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]