Difference between revisions of "Part:BBa K2675011"

 
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<partinfo>BBa_K2675011 short</partinfo>
 
<partinfo>BBa_K2675011 short</partinfo>
  
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Synthetic RBS designed for AimP of Phi3T
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This part is a synthetic RBS designed for AimP, the immature arbitrium peptide of phage phi3T ([[Part:BBa_K2675001|BBa_K2675001]]) using the [https://salislab.net/software/forward Salis Lab RBS calculator v2.0] [1, 2].
  
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===Usage and Biology===
 
===Usage and Biology===
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This RBS was used to drive the expression of AimP ([[Part:BBa_K2675001|BBa_K2675001]]) under the control of the ([[Part:BBa_J23100|BBa_J23100]]) promoter in the composite part ([[Part:BBa_K2675041|BBa_K2675041]]).
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Using the  Salis Lab RBS calculator v2.0 [1, 2], the predicted features of this RBS are:
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Translation Initiation Rate (au) : 36520,42
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dG_total (kcal/mol) : -7,53
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dG_mRNA_rRNA (kcal/mol) : -11,61
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dG_spacing (kcal/mol) : 0
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dG_standby (kcal/mol) : 0
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dG_start (kcal/mol) : -2,76
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dG_mRNA (kcal/mol) : -7,62
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Accuracy (warnings) : OK
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Revision as of 13:44, 7 October 2018


Synthetic RBS designed for AimP of Phi3T

This part is a synthetic RBS designed for AimP, the immature arbitrium peptide of phage phi3T (BBa_K2675001) using the Salis Lab RBS calculator v2.0 [1, 2].

Usage and Biology

This RBS was used to drive the expression of AimP (BBa_K2675001) under the control of the (BBa_J23100) promoter in the composite part (BBa_K2675041). 

Using the  Salis Lab RBS calculator v2.0 [1, 2], the predicted features of this RBS are:
Translation Initiation Rate (au)	:	36520,42
dG_total (kcal/mol)	:	-7,53
dG_mRNA_rRNA (kcal/mol)	:	-11,61
dG_spacing (kcal/mol)	:	0
dG_standby (kcal/mol)	:	0
dG_start (kcal/mol)	:	-2,76
dG_mRNA (kcal/mol)	:	-7,62
Accuracy (warnings)	:	OK


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]