Difference between revisions of "Part:BBa K2547000"
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This part is the coding sequence (CDS) of human carbonic anhydrase (CA-II), because Carbonic Anhydrase II (CA2) is a metalloenzyme that can efficiently catalyze the formation of HCO3- from CO2 and H2O. This reaction is highly efficient with H+. The reaction rate is extremely fast, and the histidine tag (His-Tag) is linked to the coding sequence (CDS) to facilitate the purification of carbonic anhydrase protein. | This part is the coding sequence (CDS) of human carbonic anhydrase (CA-II), because Carbonic Anhydrase II (CA2) is a metalloenzyme that can efficiently catalyze the formation of HCO3- from CO2 and H2O. This reaction is highly efficient with H+. The reaction rate is extremely fast, and the histidine tag (His-Tag) is linked to the coding sequence (CDS) to facilitate the purification of carbonic anhydrase protein. | ||
− | <div align="center"><img src="https://static.igem.org/mediawiki/parts/c/c9/T--AHUT_China--_shengwu1235.jpg" width=" | + | <div align="center"><img src="https://static.igem.org/mediawiki/parts/c/c9/T--AHUT_China--_shengwu1235.jpg" width="100" height="252" alt=""/></div> |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 13:21, 7 October 2018
Carbonic anhydrase 2
This part is the coding sequence (CDS) of human carbonic anhydrase (CA-II), because Carbonic Anhydrase II (CA2) is a metalloenzyme that can efficiently catalyze the formation of HCO3- from CO2 and H2O. This reaction is highly efficient with H+. The reaction rate is extremely fast, and the histidine tag (His-Tag) is linked to the coding sequence (CDS) to facilitate the purification of carbonic anhydrase protein.
<img src="" width="100" height="252" alt=""/>
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]