Difference between revisions of "Part:BBa K2570010:Design"

 
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===References===
 
===References===
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Wang, P., Yang, X., Lin, B., Huang, J., & Tao, Y. (2017). Cofactor self-sufficient whole-cell biocatalysts for the production of 2-phenylethanol. Metabolic Engineering, 44(August), 143–149. https://doi.org/10.1016/j.ymben.2017.09.013

Revision as of 11:14, 7 October 2018


Production of 2-PE: Aro10+PAR+TyrB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2242
    Illegal NotI site found at 2605
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 4044
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Glutamate dehydrogenase plays a key role in 2-PE biosynthesis.


Source

The phenyl pyruvate decarboxylase from S. cerevisiae (Aro10)

https://www.ncbi.nlm.nih.gov/protein/NP_010668.3?report=genbank&log$=protalign&blast_rank=1&RID=V0V04K3B015

the phenyla-cetaldehyde reductase from the rose (PAR)

https://www.ncbi.nlm.nih.gov/protein/BAG13450.2?report=genbank&log$=protalign&blast_rank=1&RID=V0NV9P35014

The aromatic transaminase from E. coli (TyrB)

https://www.ncbi.nlm.nih.gov/protein/WP_032305522.1?report=genbank&log$=protalign&blast_rank=3&RID=V13P17MW014


References

Wang, P., Yang, X., Lin, B., Huang, J., & Tao, Y. (2017). Cofactor self-sufficient whole-cell biocatalysts for the production of 2-phenylethanol. Metabolic Engineering, 44(August), 143–149. https://doi.org/10.1016/j.ymben.2017.09.013