Difference between revisions of "Part:BBa R0040:Experience"
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| + | <partinfo>BBa_R0040 AddReview 5</partinfo><br> | ||
| + | <I>[[User:Rshetty|Reshma Shetty]]</I> | ||
| + | |width='60%' valign='top'| | ||
| + | <partinfo>BBa_R0040</partinfo>, when used to regulate transcription of GFP in <partinfo>BBa_I7100</partinfo>, demonstrated the expected behavior. <partinfo>BBa_R0040</partinfo> also worked well in the constitutive wintergreen odor generator (<partinfo>BBa_J45120</partinfo>) and banana odor generator (<partinfo>BBa_J45200</partinfo>). | ||
| + | |} | ||
<!-- DON'T DELETE --><partinfo>BBa_R0040 EndReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_R0040 EndReviews</partinfo> | ||
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| + | ==Characterization== | ||
| + | [[Image:MITiGEM2006transcriptionalcontroldevices.png|thumb|left|600px| | ||
| + | '''Growth phase dependent transcriptional control devices'''<br> | ||
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| + | We successfully designed, constructed and tested transcriptional control devices for constitutive, stationary phase dependent and exponential phase dependent protein production (A-C). To test and verify function of our three transcriptional control devices, we assembled each control device with the GFP protein generator <partinfo>BBa_E0840</partinfo> and monitored the fluorescence of ''E. coli'' cultures with each device over time. For each device, we plot the change in fluorescence per unit time (normalized GFP synthesis rate) versus the cell density (OD600nm) (D). The constitutive transcriptional control device produced a high GFP synthesis rate irrespective of cell density. The stationary phase transcriptional control device produced a low initial GFP synthesis rate which increased with culture cell density. The exponential phase transcriptional control device produced an initially high GFP synthesis rate which dropped off as cell density increased. Data shown are averages of triplicate measurements of cultures grown from three individual colonies of each device. Error bars are the standard deviation of the three individual cultures. | ||
| + | ]] | ||
Revision as of 00:07, 8 March 2008
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_R0040
Used this part to create constituitively on GFP and constituitively on OriTf, OriTr. This was one of our most-often used and reliable parts. Have not tried to de-activate it using TetR yet. Worked very well with our parts. [smelissali 6/7/06]
User Reviews
UNIQe79698d8ea1e4ed8-partinfo-00000000-QINU
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Antiquity |
This review comes from the old result system and indicates that this part worked in some test. |
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smelissali |
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BBa_R0040, when used to regulate transcription of GFP in BBa_I7100, demonstrated the expected behavior. BBa_R0040 also worked well in the constitutive wintergreen odor generator (BBa_J45120) and banana odor generator (BBa_J45200). |
UNIQe79698d8ea1e4ed8-partinfo-00000009-QINU
Characterization
Growth phase dependent transcriptional control devices
We successfully designed, constructed and tested transcriptional control devices for constitutive, stationary phase dependent and exponential phase dependent protein production (A-C). To test and verify function of our three transcriptional control devices, we assembled each control device with the GFP protein generator BBa_E0840 and monitored the fluorescence of E. coli cultures with each device over time. For each device, we plot the change in fluorescence per unit time (normalized GFP synthesis rate) versus the cell density (OD600nm) (D). The constitutive transcriptional control device produced a high GFP synthesis rate irrespective of cell density. The stationary phase transcriptional control device produced a low initial GFP synthesis rate which increased with culture cell density. The exponential phase transcriptional control device produced an initially high GFP synthesis rate which dropped off as cell density increased. Data shown are averages of triplicate measurements of cultures grown from three individual colonies of each device. Error bars are the standard deviation of the three individual cultures.
We successfully designed, constructed and tested transcriptional control devices for constitutive, stationary phase dependent and exponential phase dependent protein production (A-C). To test and verify function of our three transcriptional control devices, we assembled each control device with the GFP protein generator BBa_E0840 and monitored the fluorescence of E. coli cultures with each device over time. For each device, we plot the change in fluorescence per unit time (normalized GFP synthesis rate) versus the cell density (OD600nm) (D). The constitutive transcriptional control device produced a high GFP synthesis rate irrespective of cell density. The stationary phase transcriptional control device produced a low initial GFP synthesis rate which increased with culture cell density. The exponential phase transcriptional control device produced an initially high GFP synthesis rate which dropped off as cell density increased. Data shown are averages of triplicate measurements of cultures grown from three individual colonies of each device. Error bars are the standard deviation of the three individual cultures.