Difference between revisions of "Part:BBa K1592002:Design"
(Key point in design is conforming primer sequence) |
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===References=== | ===References=== | ||
+ | //It's a short plasmids with known seuence.So we choose PCR to realize the point mutation. we design forward primer as cgaggccgctgctggtgctcacgccactgccggtgccattg and reverser primer as caatggcaccggcagtggcgtgagcaccagcagcggcctcg.Then it's easy to do following steps: running gel, gel extraction, sequencing |
Latest revision as of 08:41, 7 October 2018
Yarrowia lipolytica cell wall protein 3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 14
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 35
Design Notes
No
Source
The source of the part will be its sequence which was retrieved from GenBank, and altered some regions by our lab.
References
//It's a short plasmids with known seuence.So we choose PCR to realize the point mutation. we design forward primer as cgaggccgctgctggtgctcacgccactgccggtgccattg and reverser primer as caatggcaccggcagtggcgtgagcaccagcagcggcctcg.Then it's easy to do following steps: running gel, gel extraction, sequencing