Difference between revisions of "Part:BBa K2794001"
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|'''Function''' | |'''Function''' | ||
| − | |Exosome Membrane | + | |Exosome Membrane Anchoring |
|- | |- | ||
|'''Use in''' | |'''Use in''' | ||
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CD63 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth, and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. | CD63 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth, and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. | ||
| − | CD63 (varient 1) passes through the membrane 4 times. The N- and C- terminus is both located inside the membrane. The protein has | + | CD63 (varient 1) passes through the membrane 4 times. The N- and C- terminus is both located inside the membrane. The protein has two extracellular cringle (EC1, EC2) and one intracellular loop (IL), with EC2 significantly longer than EC1 and carries main cellular functions. |
| + | |||
| + | [[File:T--SHSU_China--CD63_hand.png|700px|]] | ||
CD63 is recently researched and used in the field of extracellular vesicle (EVs) research. Due to its strong expression on EVs, it is used in exosome identification, extraction and targeted therapy. A protein sequence can be attached to either the N- or C- terminus so that a protein cargo can be loaded into exosomes using CD63. () | CD63 is recently researched and used in the field of extracellular vesicle (EVs) research. Due to its strong expression on EVs, it is used in exosome identification, extraction and targeted therapy. A protein sequence can be attached to either the N- or C- terminus so that a protein cargo can be loaded into exosomes using CD63. () | ||
Revision as of 08:09, 7 October 2018
CD63 Varient 1 (Homo Sapiens)
| CD63 Varient 1 (Homo Sapiens) | |
|---|---|
| Function | Exosome Membrane Anchoring |
| Use in | Mammalian cells |
| RFC standard | RFC 10 compatible |
| Backbone | pSB1C3 |
| Submitted by | [http://2018.igem.org/Team:SHSU_China SHSU_China] |
Overview
CD63 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth, and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker.
CD63 (varient 1) passes through the membrane 4 times. The N- and C- terminus is both located inside the membrane. The protein has two extracellular cringle (EC1, EC2) and one intracellular loop (IL), with EC2 significantly longer than EC1 and carries main cellular functions.
CD63 is recently researched and used in the field of extracellular vesicle (EVs) research. Due to its strong expression on EVs, it is used in exosome identification, extraction and targeted therapy. A protein sequence can be attached to either the N- or C- terminus so that a protein cargo can be loaded into exosomes using CD63. ()
Design
Experience
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References