Difference between revisions of "Part:BBa K2549005:Design"
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===Design Notes===
 
===Design Notes===
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Its heavy-chain variable region(αCD19a) and light-chain variable region(αCD19b) are fused using a glycine-rich peptide linker(3 repeats of GGGGS, or G4S)[1].
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The heavy-chain variable region (&#945;CD19a) and light-chain variable region (&#945;CD19b) were fused together using a glycine-rich peptide linker(3 repeats of GGGGS, or G4S)<ref>Chimeric Antigen Receptor-Modified T Cells in Chronic Lymphoid Leukemia; Chimeric Antigen Receptor-Modified T Cells for Acute Lymphoid Leukemia; Chimeric Antigen Receptor T Cells for Sustained Remissions in Leukemia. N Engl J Med, 2016 Mar;374(10):998  PMID: 26962747; DOI: 10.1056/NEJMx160005</ref>.
  
 
===Source===
 
===Source===
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===References===
 
===References===
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[1]Chimeric Antigen Receptor-Modified T Cells in Chronic Lymphoid Leukemia; Chimeric Antigen Receptor-Modified T Cells for Acute Lymphoid Leukemia; Chimeric Antigen Receptor T Cells for Sustained Remissions in Leukemia.
 
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N Engl J Med, 2016 Mar;374(10):998  PMID: 26962747; DOI: 10.1056/NEJMx160005
 

Revision as of 05:24, 7 October 2018


anti-CD19


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 444


Design Notes

The heavy-chain variable region (αCD19a) and light-chain variable region (αCD19b) were fused together using a glycine-rich peptide linker(3 repeats of GGGGS, or G4S)[1].

Source

from IDT(gBlock), codon optimized for human

References

  1. ↑ Chimeric Antigen Receptor-Modified T Cells in Chronic Lymphoid Leukemia; Chimeric Antigen Receptor-Modified T Cells for Acute Lymphoid Leukemia; Chimeric Antigen Receptor T Cells for Sustained Remissions in Leukemia. N Engl J Med, 2016 Mar;374(10):998 PMID: 26962747; DOI: 10.1056/NEJMx160005