Difference between revisions of "Part:BBa K2541001"
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<h1>'''Characterization'''</h1>
 
<h1>'''Characterization'''</h1>
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The thermosensor is constructed on the pSB1C3 vector by goldengate assembly method. Ii is composed of Anderson promotor (BBa_J23104), thermosensor (BBa_K2541001) and sfGFP_optimism(BBa_K2541400). The following device is used to characterize the thermosensor. Then we tranfform the device to E.coli DH5a. We measured the sfGFP_optimism expression to get the actual sensitive temperature of the heat induced RNA thermosensor.
  
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As shown in the figure, the thermosensor sensitive temperature range is [  ].
 
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Revision as of 05:08, 7 October 2018


Heat-inducible RNA-based thermosensor-1

An RNA thermosensor that can be used for temperature sensitive post-transcriptional regulation which is based on the change of RNA sencondary structure.The heat induced RNA thermosensors can initiate translation of downstream genes at high temperature.

Usage and Biology


Characterization

The thermosensor is constructed on the pSB1C3 vector by goldengate assembly method. Ii is composed of Anderson promotor (BBa_J23104), thermosensor (BBa_K2541001) and sfGFP_optimism(BBa_K2541400). The following device is used to characterize the thermosensor. Then we tranfform the device to E.coli DH5a. We measured the sfGFP_optimism expression to get the actual sensitive temperature of the heat induced RNA thermosensor.


As shown in the figure, the thermosensor sensitive temperature range is [ ].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]